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Process Development Associate , Vectors

Company: Yoh, A Day & Zimmermann Company
Location: Brookline
Posted on: February 22, 2021

Job Description:

Process Development Scientist ( Downstream, Bioprocess, Viral Vectors, AAV) 305174

RESPONSIBILITIES:


Bioprocess;
Downstream Process Development, process optimization for manufacturing viral vectors
Vector Engineering/ Viral Vectors. Support vector core Vector Engineering processes, Process Development
Support vector core vector engineering processes, process development, and process optimization
Process Development, process optimization, Bioprocess
Upstream/ Downstream Process Development, process optimization for manufacturing viral vectors
Bioprocess development of AAV vectors focusing, upstream cultivation and harvest conditions to optimize yield
Working with AAV viral particles, fermentation, plasmid recovery, purification and formulation, sterilization, and harvesting process steps
Apply research/ industry knowledge of Lentivirus (LV) and or Adeno Associated Virus (AAV)
Cell Line maintenance, Cell Line development, Media development; as cell are transfected with plasmids to generate viral vectors, which are efficiently harvested
Creating and maintaining stable cell lines
Troubleshooting and optimizing stepwise.
Plasmid Preparation, Buffer, Media Preparation, Aseptic Processing, growth & maintenance of cell lines.
Handling Bioreactors, reagent/media prep, sterile manufacturing, upstream/downstream processing
Transfection, generate viral vectors, and efficient harvesting
Troubleshooting and optimizing processes stepwise.
Apply skills in cGMP and gene therapy, vector purification, process scale-up, and sterile manufacturing
Bioprocess development of AAV vectors focusing, upstream cultivation and harvest conditions to optimize yield
Managing suspension cultures, downstream purification recoveries, and vector potency
Overall support of upstream process development for novel gene therapy vector.








REQUIREMENTS:


Bachelor's (BS) or Master's (MS) in Molecular Biology, Virology, Biology, Biochemistry or related
Minimum of a 4-8 yrs. + experience in the Biotech, Cell & Gene Therapy (CGT) industry working with viral vectors
In depth knowledge of both upstream processing and or downstream processing
Knowledge of protein purification, mammalian cell culture, virology, viral validation, Preformulation
Formulation, ultracentrifugation, flocculation, scale-able chromatography and tangential flow filtration (TFF).
Process Development (PD), manufacturing of viral vectors; transfection, transcription, translation, AAV vector
in vivo genetic modification, Plasmid DNA Extraction
4-8 yrs. + experience handling bioreactors, media development, and process development/ bioprocess refinement
4-8 yrs. + experience in protein and gene therapy, vector purification, process scale-up, and sterile manufacturing
Upstream/Downstream and the equipment and tools used in that process including; bioreactors, Vi-CELL, S.U.M.'s, sterile tubing welders, filters, balances, microscopes, pipettes, CellSTACK's, HYPERstack's Clipsters, DeltaV, BSC's, pH Meters, Osmometers, Autoclaves etc.
Sterile manufacturing, cGMP, cleanroom, Upstream / Downstream Process Development and GMP compliance.
UPSTREAM - 20L-200L bioreactors, cell expansion, transient transfection, viral production and clarification
DOWNSTREAM -Recovery and purification of viral vectors and removal of contaminants. Nuclease digestion, Purification, Sterile Filtration and Fill
ANALYTICS - PAT, feed rates, pH control, Potency measurement, Bioburden, Mycoplasma, Endotoxin
cGMP, sterility, fermentation, mammalian cell culture, bioprocess optimization, purification, including clarification by filtration/ centrifugation; chromatography, ion exchange, sizing, multi-modal and other resins
Tangential Flow Filtration (TFF) or Ultracentrifugation/ Diafiltration (UF/DF), Size Exclusion Chromatography.





PLEASE:


Share your updated resume using E-MAIL ADDRESS, philip.cooke@yoh.com
CALL 860-405-7181 (anytime up until 7pm) to discuss











#ZipSPG

#LI-PC

Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. Visit https://www.yoh.com/applicants-with-disabilities to contact us if you are an individual with a disability and require accommodation in the application process.PandoLogic.Category: Science, Keywords: Biochemist

Keywords: Yoh, A Day & Zimmermann Company, Brookline , Process Development Associate , Vectors, Other , Brookline, Massachusetts

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